Methods and fiber-optic spectrometry system for control of photosensitizer in tissue during photodynamic therapy

Photodynamic therapy used in cancer treatment as an alternative or combined with conventional treatments tumors. PDT was involved three key components: a photosensitizer, a light source and tissue oxygen. The combination of these three components leads to the chemical destruction of any tissues which have either selectively taken up the photosensitizer or have been locally exposed to light. The data about absorption spectra properties of the photosensitized tumor tissues in vivo are very important for the evaluation photosensitizer concentration and influence of the chemical environment on photosensitizer properties in tissue. Determination of maximum photosensitizer concentration timeframe is necessary condition for effective PDT. Spectrometric equipment supplied with proper fiber-optic probes and small semiconductor laser can be successfully used for photosensitizer concentration determination and monitoring in tissue.