Contouring of microcapillary images based on sharpening to one-pixel width of boundary curves

The paper presents a new sharpening method that can be applied for the low-contrast medical images as well as general edge detection. The method provides more reliable diagnosis of the vascular system based on image processing. The new contouring method was compared with Sobel, low-frequency filtration and Canny edge detection methods on the example images with micro-capillaries.